The question was asked for manual installation with virtual env, not for All-In-One. The instructions for autostart, located here:
should be updated for virtual environment installations, which is the recommended installation method. On another subject, it would help a lot if there was a search ability on the home assistant site. It took me a long time just to find the autostart instructions in the first place, and then when I did, I found them to be confusing, because venv was not mentioned.