USB Boot on Raspberry Pi 4

I have been able to boot my Rpi4 HA installation (installation described here) from a USB flash drive for sometime. Originally I needed to use a SD card for the boot process but with the FW update this card is no longer needed

Recently I installed HA ( This version as well as This version) on a flash drive. Neither of these versions would boot using the same Rpi4 as above.

Is there another step I have to do to get either of these versions to boot? Bear in mind I do have the Rpi4 booting this version without issue.